Test sample positioning system

ABSTRACT

A positioning system for a sample testing machine includes a base pan and a set of independent drive systems for sliding a sample tray across the base pan. Each of the drive systems includes a paddle that engages a side of the sample tray. The paddle is slideably mounted to a shaft with a collar that slides over the shaft. A motor and drive belt is provided to move the collar along the shaft, causing the paddle to engage the side of the sample tray and slide the sample tray across the base pan. Track sections are provided in the base pan to prevent the sample tray from rotating as the paddle slides the sample tray across the base pan.

BACKGROUND OF THE INVENTION

A. Field of the Invention

This invention relates to systems and methods for moving a test sampleor group of test samples to various stations of a machine, such as abiological sample testing machine.

B. Description of Related Art

Biological samples can be subject to chemical or optical analysis usingvarious techniques, one of which is transmittance and/or fluorescenceoptical analysis. The purpose of the analysis may be to identify anunknown biological agent in the sample, test the sample to determine theconcentration of a substance in the sample, or determine whether thebiological agent is susceptible to certain antibiotics, as well as theconcentration of antibiotics that would be effective in treating aninfection caused by the agent.

A technique has been developed for conducting optical analysis ofbiological samples that involves the use of a sealed test sample cardcontaining a plurality of small sample wells. Typically, duringmanufacture of the cards e.g. for microbiological analysis, the wellsare filled with either various types of growth media for variousbiological agents, or else various concentrations of differentantibiotics. The cards have an internal fluid passageway structure forallowing fluid to enter the wells of the card through a transfer tubeport, and an integral transfer tube extending outwardly from thetransfer tube port. The prior art method involved the manual insertionof one end of the transfer tube into the card and the other end into atest tube, and then the manual placement of the card with attachedtransfer tube and test tube into a vacuum filling and sealing machine,such as the Vitek® Filler Sealer. The filling and sealing machinegenerates a vacuum, causing the fluid in the test tube to be drawn intothe wells of the sample card.

After the wells of the card are loaded with the sample, the cards aremanually inserted into a slot in a sealer module in the machine, wherethe transfer tube is cut and melted, sealing the interior of the card.The cards are then manually removed from the filler/sealer module andloaded in a column fashion into a reading and incubating machine, suchas the VITEK® Reader. The reading and incubating machine incubates thecards at an incubation temperature, and has an optical assembly thatmoves up and down relative to the cards to read the wells of the cards.

This arrangement has limitations, in that two machines are required toprocess and analyze the cards. Furthermore, additional time and laborare required to conduct the complete analysis of the card.

To combine the several functions of biological sample processing andoptical reading into a single automatic sample processing and readingmachine poses substantial challenges. One particularly difficultchallenge is to provide a positioning system to move the samples aboutthe machine from station to station. The present invention provides apositioning system that is reliable, precise in its positioning of thesamples, and that is suitable for the varied types of operationsperformed on automated sample testing equipment such as test samplecards.

Representative prior art test sample positioning systems for use inbiological and immunoassay analyzer machines are described in U.S. Pat.No. 4,265,855 to Mandle et al., U.S. Pat. No. 5,232,665 to Burkovich etal., U.S. Pat. No. 4,890,930 to Nohso, U.S. Pat. No. 4,710,352 to Slateret al., U.S. Pat. No. 4,632,808 to Yamamoto et al., and U.S. Pat. No.4,236,825 to Gilford. These systems tend to either have unnecessarycomplexity in their mechanical components or involve drive and transportsystems that are unsuitable to the processing of test sample cards andthe associated test tubes. The system described in the above-citedMandle et al. patent discloses a drive pin arrangement for moving a setof blocks carrying test tubes about an immunochemical analysis machine.The machine is fully loaded with blocks positioned in front and rearrows, with a vacant spot for one block at opposite corners of themachine. Drive pins then move simultaneously to push one block from thefront row to the vacant spot on the back row, and one block from theback row to a vacant spot on the front row. The blocks are then advancedone space along the length of the front and rear rows, and the processrepeats. The functioning of this drive system is dependent on themachine being filled with blocks, and hence lacks flexibility. Further,in order for one block to be moved through all the stations of themachine, the blocks in front of it must first be cycled through thestations around the machine.

The present invention overcomes the disadvantages of the prior artpositioning systems by providing an efficient test sample positioningsystem for that is suitable for biological and chemical sample testingmachines. It provides independent drive systems that can simultaneouslytransport a number of trays loaded with samples independently,permitting processing by the various stations of the trays at the sametime, without the delays and complexities found in many prior artsystems. The invention further permits the performance of the functionsof loading sample fluids into the cards and reading of the cards in asingle machine automatically and without human intervention, thusachieving a significant advance in the art. These and other objects,advantages and features of the invention will become more apparent fromthe following detailed description of a preferred embodiment of theinvention.

SUMMARY OF THE INVENTION

A positioning system is provided for moving a test sample tray across abase pan to various stations of a sample testing machine. The systemcomprises a set of independent drive systems which operate to move thesample tray independently from each other. Each of the drive systemsincludes a paddle that engages the side of the sample tray. The paddleis mounted to a collar that is moveable along a rotatable shaft by adrive motor and drive belt subassembly. As the drive motor and drivebelt subassembly moves the collar along the shaft, the paddle pulls thesample tray across the base pan. Preferably, the drive systems alsoinclude a shaft rotation motor for rotating the shaft such that thepaddle is rotatable into and out of engagement with the sample tray.

The base pan may be given a rectangular shape, in which case fourindependent drive systems may be provided, enabling the tray to be movedalong all four of the sides of the base pan. By making the drive systemsoperable independently from each other, the positioning system allowsfor a plurality of sample trays to be moved about the machine at thesame time.

The base pan is preferably provided with a set of track sections definedby a set of raised ridges and a raised rim of the base pan. The tracksections prevent the boat from undergoing any rotational motion as theboat is pulled by the drive systems along the base pan. The tracksections have a set of slots to permit the feet of the sample tray topass through the track sections as the sample tray is moved sequentiallyin perpendicular directions across the base pan.

BRIEF DESCRIPTION OF THE DRAWINGS

Presently preferred embodiments of the invention are depicted in thedrawings, wherein like reference numerals refer to like elements in thevarious views, and wherein:

FIG. 1 is a perspective view of a preferred automatic biological sampletesting machine that incorporates the test sample positioning system ofthe present invention;

FIG. 2 is a perspective view of the machine of FIG. 1, with the dilutingand pipetting stations removed to better illustrate the vacuum stationof the machine;

FIG. 3 is a detailed perspective view of the test sample positioningsystem of FIGS. 1 and 2;

FIG. 4 is a plan view of the base pan of FIGS. 1-3;

FIG. 5 is a detailed perspective view of the boat and cassette of FIGS.1-3;

FIG. 6 is a plan view of the cassette and boat of FIG. 5 in an assembledcondition;

FIG. 7 is a side view of the cassette and boat of FIG. 6;

FIG. 8 is a sectional view of the cassette and boat of FIG. 7 along thelines 8--8;

FIG. 9 is a side view of the cassette and boat of FIG. 5, as seen fromthe opposite side of FIG. 7, showing the apertures in the cassette thatreceive touch memory buttons that store information as to the cardscarried by the cassette;

FIG. 10 is a bottom plan view of the boat of FIGS. 1 and 5-9;

FIG. 11 is a detailed top view of the paddle 38A engaging the boat 22;

FIG. 12 is a sectional view of the paddle and collar of FIG. 11; and

FIG. 13 is a perspective view of the fourth drive assembly of FIG. 3,showing the fourth paddle 38D and other components of the assembly.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

Overview of Preferred Test Sample Machine

FIG. 1 is a perspective view of a preferred biological sample testingmachine 20. The following detailed description of the preferredembodiment of the inventive optical reader and sample card transportsystems will be discussed in the context of the biological sampletesting machine 20. It will be appreciated, however, that the principlesof the invention may be used in other types of testing machines besidesthe preferred biological sample testing machine 20. Other possibleimplementations include chemical testing, immunochemistry,immunodiagnostic, and nucleic acid probe assay machines.

The biological sample testing machine 20 includes a biological testsample positioning system 100 having four independent motor-drivenpaddles, which is designed to pull a sample tray 22 (referred to hereinas a "boat") across a base pan 24 around the machine 20 to severaldiscrete stations, where various operations are performed on the samplesin the boat 22. Prior to the start of the procedure, a technician loadsa cassette 26 with a plurality of test cards 28 and receptacles such astest tubes 30 containing biological or control samples to be tested.Each test card 28 has an L-shaped transfer tube 32 protruding therefromfor permitting the fluids containing biological samples to be drawn fromthe test tubes 30 into the wells of the test cards 28. The technicianplaces the loaded cassette 26 into the boat 22 at a loading station forthe machine, such as the front, right hand corner of the base pan 24shown in FIG. 1. The combined boat 22 and loaded cassette 26 are thenmoved as a unit over the surface of the base pan 24 about the machine 20by the test sample positioning system 100. In a typical microbiologicaltesting scenario, described below for purposes of illustration but notlimitation, the test cards 28 come in two varieties: (1) identificationcards, in which particular different growth media are placed in thewells of the card 28 when the cards are manufactured, and (2)susceptibility cards, in which different concentrations of differentantibiotics are placed in the wells of the card 28. The identificationcards are used to identify the particular unknown biological agentpresent in the sample. The susceptibility cards are used to determinethe susceptibility of the biological agent to various concentrations ofantibiotics or other drugs. In the test procedure described below,identification and susceptibility tests can be performed on a singlesample in one cycle of operation of the machine 20. To accomplish this,the cassette 26 is loaded such that a test tube 30A containing abiological sample, connected via a transfer tube 32 to an identificationcard 28A, is placed adjacent to a test tube 30B connected via a transfertube 32 to a susceptibility card 28B. Alternate test cards may beprovided for other types of biological or chemical testing, such cardsincorporating various test reagents.

The cards 28 preferably contain bar codes across the top of the card forreading by a bar code reader built into the machine 20. The bar codesare unique to each card, and identify card information such as cardtype, expiration date, and serial number, and are used to correlate testdata and/or results from the cards with the patient and the biologicalsample. In addition, the entire boat or cassette may have sampleinformation for all of the cards loaded in the cassette stored on amemory device affixed to the cassette 26, such as a memory button or"touch button" available from Dallas Semiconductor Corp., 4401 S.Beltwood Parkway, Dallas Tex.

In the representative example shown in FIG. 1, seven or eight of thetest tubes 30 in the boat 22 contain biological samples, and are influid communication with identification cards 28 by the straw-liketransfer tube 32. The biological sample test tube 30A and its associatedidentification card 28A can be thought of as a set. The biologicalsample test tubes and identification cards are typically arranged in analternating pattern in the cassette 26. Each biological sample test tube30A and identification card 28A set is adjacent to an empty test tube30B placed in communication with a susceptibility card 28B via atransfer tube 32. It will be appreciated that the cards and associatedtest tubes could be ordered in any order in the cassette 26 depending onthe particular testing requirements for the samples. For example, thecards could be arranged as follows: identification (ID), susceptibility(SU), ID, ID, ID, SU, SU, ID, SU . . . . Further examples would be allidentification cards and all susceptibility cards.

After the loaded cassette 26 is installed at the loading station on tothe boat 22, the test sample positioning system 100 operates to move theboat 22 over the base pan past a station 84 (FIG. 1) for achievingcassette information stored in touch buttons applied to the rear of thecassette 26. The test sample positioning system 100 then operates tomove the boat 22 and cassette 26 past a bar code reading station forreading bar codes applied to the tops of the cards. As the cassettepasses by the wheel 94, the wheel 94 rocks the cards 28 backwardsslightly so that the bar codes can be read by an optical bar codereader. The reader (not shown) is mounted to the center mount 34 aboveand slightly forward of the wheel 94.

The test sample positioning system 100 operates to move the boat 22 andcassette 26 over the base pan 24 to a diluting station 200. The dilutingstation contains a rotating shot tube 202, by which a predeterminedvolume of diluent (such as saline solution) is added to the emptysusceptibility test tubes in the cassette 26, e.g. test tube 30B. As theleading edge of the boat 22 is moved to the left during this process, itpasses under a pipetting station 300. The pipetting station 300 includesa mechanism that automatically removes a pipette 302 from a source ofpipettes 304, lowers the pipette 302 into the biological sample testtube 30A, and removes with vacuum a predetermined volume of biologicalfluid from the biological sample test tube 30A using the pipette 302.The pipetting and diluting stations are described in further detail in apatent application of Bishop et al. filed concurrently, Ser.No.08/60/461 filed , Feb. 21, 1996, pending which is incorporated byreference herein.

The test sample positioning system 100 then moves the boat 22 to theleft by an amount equal to the separation distance in the cassette 26between adjacent test tubes 30A and 30B, e.g. 15 ram. The pipettingstation 300 then lowers the pipette 302 containing the biological fluidfrom the biological sample test tube 30A into the adjacentsusceptibility test tube 30B (having already received a quantity ofdiluent from the diluting station 200), expels the fluid into the testtube 30B, and drops the pipette 302 into the susceptibility test tube30B. The process of movement of the boat 22 by the test samplepositioning system 100, adding diluent to the susceptibility test tubes30B at the diluting station 200, and transferring of biological samplesfrom the biological sample test tubes 30A to the adjacent susceptibilitytest tubes 30B at the pipetting station 300, continues until all of theidentification and/or susceptibility test tubes sets (if any) in theboat 22 have been so processed. By virtue of the close spacing of thepipetting station 300 and the diluting station 200, simultaneousdiluting and pipetting operations can be performed on multiple testtubes in a single boat 22. After the last pipetting operation has beenperformed, the test sample positioning system 100 then moves the boatall the way to the left-hand edge of the base pan 24.

It will be understood by persons skilled in the art that the cassette 26may be loaded entirely with biological samples in the test tubes 30 andidentification cards 28, such as the case where a batch of biologicalsamples are to be tested to identify the contents of the samples. Inthis example, the diluting and pipetting operations are not necessary.However, in other types of sample testing, other diluents or fluids maybe added to or withdrawn from the test tubes. In the example of where nodiluting or pipetting operations are performed (e.g., where thepipetting and diluting operations were performed off-line), the cassette26 is loaded with test tubes and cards, and the positioning system 100would simply move the boat 22 and loaded cassette 26 directly past thediluting station 200 and the pipetting station 300 without stopping, allthe way to the left hand edge of the base pan 24.

Once at the left hand edge of the base pan 24, the test samplepositioning system 100 operates to move the boat 22 along the left handedge to a vacuum station 400. The vacuum station 400 is seen better inFIG. 2, which is a perspective view of the machine 20 with the dilutingstation 200 and the pipetting station 300 removed. At the vacuum station400, a vacuum chamber 402 is lowered onto the boat 22 such that thebottom surface of the vacuum chamber 402 sealingly engages the topperipheral surface 23 of the boat 22. Vacuum is applied to the chamber402 under microprocessor control, causing air in the interior of thetest sample cards 28 to evacuate out of their associated test tubes andto be withdrawn from the chamber 402. The vacuum cycle is preciselymanaged to optimize filling using a closed loop servo system to regulatethe change of vacuum and timing of the complete cycle. After apredetermined dwell period, the chamber 402 is vented gradually toatmosphere under microprocessor control. The venting of the cards causesthe fluid in the test tubes 30 to be drawn into the cards 28, fillingthe wells in the cards 28.

The test sample positioning system 100 then operates to advance the boat22 to the right across the rear of the base pan 24 to a cut and sealstation 500, located behind the center mount 34 in FIGS. 1 and 2. Thecut and seal station 500 consists of a hot cutting wire and attachedsupport plate (not shown), and a drive mechanism 502 that lowers thecutting wire and support plate to the same elevation as the top portionof the transfer tubes 32 adjacent to where the transfer tubes 32 enterthe test cards 28. As the boat 22 is advanced past the cut and sealstation 500, the transfer tubes 32 are forced past the hot cutting wire.With the assistance of fore and aft constraints placed on the movementof the cards 28 by the walls of the cassette 26, and the lateralconstraints on the movement of the card 28 by the cassette and wallstructures of the machine 20, the hot cutting wire cuts the transfertubes 32 by melting of the transfer tube material as the boat 22 isadvanced past the hot cutting wire. A small stub of transfer tubematerial is left on the exterior of the card 28. The stub seals theinterior of the card 28 from the atmosphere.

The test sample positioning system 100 then advances the boat 22 acrossthe rear of the base pan 24 behind the center mount 34 to a carouselincubation station 600. A reciprocating cam driver is mounted to thecenter mount 34 opposite a slot 602 that pushes the cards off thecassette 26 one at a time through the slot 602 into a carousel 604. Thecarousel 604 is housed in an enclosure that is maintained at anappropriate incubation temperature for the particular assay, forexample, 35 degrees C. The enclosure is not fully shown in FIGS. 1 and 2in order to show the carousel 604. The carousel 604 is rotated insynchronism with the movement of the boat 22 over the rear of the basepan 24 by the test sample positioning system 100, so as to place thenext slot in the carousel 604 in line with the slot 602 opposite thenext card in the cassette 26. If the carousel 604 is only going to bepartially loaded with cards, it may be advisable to load the cards intoevery other slot a slot or two periodically in order to balance out theweight distribution in the carousel 604. For example, where the carouselhas 60 slots and only 30 cards are to be processed, the cards are loadedinto every other slot.

After all of the cards 28 have been loaded into the slots of thecarousel 604, the boat 22 is advanced along the right hand edge of thebase pan 24 back to its starting position (shown in FIGS. 1 and 2) forremoval of the cassette 26 (containing the test tubes and transfer tubesremnants) and receipt of a new cassette. Alternatively, the boat 22could be moved to an exit station located, for example, in the rear orright hand side of the base pan 24.

As the cards 28 are being incubated in the incubation station 600, thecards are periodically, sequentially pushed out of the slots of thecarousel 604 at the top of the carousel 604 by a reciprocating camdriver, one at a time, and moved by an optical scanner transport station700 past a fluorescence and transmittance optics station 800. The wellsof the card 28 are selectively subject to transmittance and/orfluorescence optical testing by the transmittance and fluorescenceoptics station 800. The transmittance and fluorescence optics station800 includes detectors and processing circuitry to generatetransmittance and fluorescence data for the wells in the cards 28, andto report the data to a central processing unit for the machine 22. Ifthe test is not complete, the transport station 700 moves the card 28back into its slot in the carousel 604 for more incubation andadditional reading.

Typically, each card will be read every 15 minutes as the carousel makesone revolution. Typical incubation times for the cards 28 are on theorder of two to eighteen hours, consisting of roughly four transmittanceand fluorescence data sets per hour for each of the wells in the card 28subject to the optical analysis.

After the testing is complete, the cards are moved by the opticalscanner transport system 700 into a card output station 900 shown inFIG. 1. The card output station 900 consists of a detachable magazine902 that is placed to the side of the optical station 800 atapproximately the same elevation as the optical station 800. Thetechnician removes the magazine 902 from the machine 20 as needed orwhen the tray is full of cards, empties the cards into a suitablebiohazard disposal unit, and replaces the magazine 902 back into themachine 20.

Test Sample Positioning System 100 Detailed Description

Referring now in particular to FIG. 3, the test sample positioningsystem 100 will be described in detail. The system 100 is shown in aperspective view in FIG. 3, with all of the stations mounted to thecenter mount 34 (such as the pipetting station 300) and the incubationstation 600 removed in order to more clearly illustrate the componentsof the positioning system 100.

The system 100 has a base pan 24 mounted to a table support structure18, across which the boat 22 is slid from station to station in themachine 20. The base pan 24 in the preferred embodiment is ofrectangular shape having four sides at right angles to each other: afront side, a left hand side (LHS), a rear side, and a fight hand side(RHS). The four sides of the base pan 24 allow the boat 22 to be movedclockwise in a loop about the machine back to its starting position at aloading station (the position of the boat 22 and cassette 26 shown inFIGS. 1-3) after all of the operations on the sample card 28 have beencompleted. However, the inventive principles of the test samplepositioning system are applicable to other geometries for a base pan 24.Additionally, the paddles and motors are capable of moving the boat 22in a counter-clockwise direction.

Referring to FIGS. 3-5, the boat 22 has four downwardly depending feet72 (FIGS. 5, 10) which fit in a pattern of track sections comprisinggrooves 36 formed between a set of raised ridges 37 and a raised rim 39extending around the perimeter of the base pan 24. The grooves 36 helpprevent any rotation of the boat 22 as the boat 22 is pulled over thebase pan 24.

When the boat 22 is initially located at the loading station, as shownin FIG. 4, the left front LF and right front RF feet 72 of the boat 22are positioned in groove 36A, the right rear (RR) foot 72 is in groove36D, with the RF foot 72 at the intersection of grooves 36A and 36D. Aplurality of slots 35 are provided in the raised ridges 37 so as topermit the feet 72 of the boat 22 to move through the ridges 36 as theboat 22 is moved about the base pan 24. For example, slot 35D permitsthe right rear RR foot 72 to move past raised ridge 37D, and slot 35Bpermits the left rear LR foot 72 to move past the ridge 37B into thegroove 36B.

In order to move the boat 22 clockwise about the base pan, fourindependent drive systems are provided for moving the boat 22. Eachdrive system moves the boat 22 in one direction along one of the foursides of the base pan 24. Referring now in particular to FIG. 3, a firstdrive system is provided for moving the boat 22 along the front edge ofthe base pan 24, and consists of a rotatable shaft 42A having a squarecross section, a collar 40A slideably mounted the shaft 42A, a drivebelt 44A mounted to the collar for sliding the collar 40A along theshaft 42A, a stepper drive motor 48A driving a belt 50A, and a pulley52A for moving the drive belt 44A back and forth along the front edge ofthe base pan, and a second pulley 46A for the drive belt 44A. A paddle38A is mounted to the collar 40A, and is provided for engaging acomplimentary surface of the boat 22 such that when the drive motor 48Ais operative to move the belt 44A such that the collar 40A is moved tothe left along the shaft 42A, the paddle 38A drags the boat 22 to theleft across the base pan 24.

A shaft rotate motor 54A is also provided with an associated belt andpulley (not shown) for rotation of the shaft 42A by an angle of 90degrees. When the shaft rotate motor 54A rotates the shaft 42A such thatthe head of the paddle 38A is in a horizontal position in the directionof the boat 22, the paddle 38A is in a position to engage acomplimentary surface on the side of the boat 22 so as to drag the boat22 as the paddle 38A and collar 40A are moved along the shaft 42A. Whenthe boat has reached the end of its travel along the front edge of thebase pan 24, the shaft rotate motor 54A rotates the shaft 42A 90 degreesin a direction such that the paddle 38A is rotated upwards away from theside of the boat 22, thereby disengaging the paddle 38A from the boat22.

Referring now in particular to FIG. 11, the paddle 38A and itsengagement with a complimentary engaging surface 60A of the boat 22 isshown in greater detail. FIG. 11 shows the boat 22 and paddle 38A inposition for movement of the boat 22 to the left along the front side ofthe base pan 24. The movement of the belt 44A by motor 48A causes thecollar 40A to slide along the shaft 42A, to the left (or to the right)depending on whether the motor 48A is operated in a forward or reversedirection. The head H of the paddle 38A pulls the boat such that theleft front foot LF slides along the groove 36A and maintains alignmentof the boat 22 relative to the base pan 24. From FIG. 11, it can be seenthat the complimentary paddle engaging surface PE comprises a surfaceoriented at an acute angle relative to the long axis of the shaft 42A.The surface PE lies in a vertical plane passing through the base pan.The acute angle is preferably between 30 and 60 degrees. The other drivesystems around the periphery of the base pan work in essentially thesame manner.

Specifically, each of the other three drive systems in the samplepositioning system 100 is functionally equivalent to the drive systemdescribed above for the front edge of the base pan 24, and each iscomposed of like components. For example, the left hand side LHS drivesystem has a shaft 42B, collar 40B with attached paddle 38B, drive beltmotor 48B, shaft rotation motor 54B etc. Like components for the rearedge of the base pan include a rotatable shaft 42C, belt drive motor48C, etc. Similarly, the right hand side (RHS) drive system has arotatable shaft 42D, collar 40D and attached paddle 38D, etc.

FIG. 12 is a sectional view of the collar and shaft of FIG. 11, showingthe rotation of the paddle into and out of engagement with the side ofthe boat 22. This rotation is accomplished by the operation of the shaftrotation motor 54A of FIG. 3 and the movement of its associated belt andpulleys. The collar 40A has a low-friction lining L surrounding theshaft 42A, which reduces the torque on the drive motor for the belt 44D.

FIG. 13 is a perspective view of the fourth drive system that operatesto pull the boat 22 along the right hand side of the base pan 24. Thedrive system includes a collar 40D that slides along the square shaft42D, and a paddle 38D. The pulley 52D for the drive motor 48D (see FIG.3) operates to move the belt 44D back and forth, sliding the collar 40Dalong the shaft 42D. The rotation belt RB for the shaft rotation motor54D (FIG. 3) serves to rotate the shaft 42D, thereby permitting thepaddle 38D to rotate into and out of engagement with the side of theboat 22.

From FIG. 3, it can be seen that after the front drive paddle 38A hasslid the boat 22 all the way to the extreme left hand side of the basepan 24, the shaft rotate motor 54A rotates the shaft 42A such thatpaddle 38A is rotated out of engagement with the boat 22. At this time,the drive motor 54A may then move the paddle 38A all the way to the endof the shaft 42A adjacent to the pulley 46A, so as to enable the paddleto engage another boat. Meanwhile, the drive motor 48B will have movedthe LHS paddle 38B to the end of the shaft 42B so as to engage the boat22 at the left front corner of the base pan 24. The LHS drive motor 48Band paddle 42B enable the boat 22 to be moved along the LHS of the basepan 24 to the vacuum station 400 (FIGS. 1 and 2), or to any otherstation that may be positioned above the base pan 24 on the LHS of thebase pan 24.

Referring in particular to FIG. 4, it is important that when the boat 22is slid along the LHS of the base pan from its position 22B at the frontleft corner (FLC) to the rear left corner (RLC), the boat not undergoany substantial rotation, indicated by the arrow R. Substantial rotationwould cause the right rear corner RR of the boat 22 to bind at thecorner 33 of the center mount 34. While the groove 36B is helpful inpreventing rotation, it has been found after experimentation that makingcorner 33 a sharp corner prevents the binding. The sharp cornerimmediately forces the boat 22 to maintain alignment in the groove 36Bas the boat is moved away from the front left corner FLC as the drivesystem moves the boat 22 along the left hand side LHS of the base pan24.

After the vacuum loading test samples into the cards 28 is complete, theLHS drive system moves the boat to the left rear corner of the base pan,and the rear drive system 38C, 40C, 42C, etc. takes over. The rear drivesystem 38C, 40C, 42C etc. moves the boat 22 into position for loading ofthe cards one at a time into the carousel of the incubation station.After the rear drive system as moved the boat 22 across the rear of thebase pan 24 to the right rear corner of the base pan 24, the RHS drivesystem 38D, 40D, 42D, etc. takes over to move the boat 22 along the RHSof the base pan.

Since the four drive systems move a boat 22 independently from eachother, it is possible for a plurality of boats 22 (e.g., four) to beprocessed simultaneously in the machine 20. In order to accommodate fourboats on the base pan 24 after the cards in the boats have been loadedinto the carousel, it may be necessary to advance the first boat to beprocessed along the front edge of the base pan to the left in order toavoid a jam of boats at the RHS of the base pan and at the loadingstation 22' (FIG. 4). It may also be necessary to permit the boats to bemoved in a counter clockwise direction if for some reason the boats 22are not positioned exactly where they need to be for the processing ofthe cards 28 by the various stations. A further possibility is toprovide a separate exit station on the rear or RHS of the base pan forboats 22 that have been processed.

The base pan 24 is preferably made out of a low friction and low wearmaterial such as structural foam. Drain holes 25A, 25B are provided onthe bottom of the base pan 24 for allowing any spilled fluids on thebase pan to be carried out of the machine 20.

An electronic control system for the motors of the test samplepositioning system 100 is not shown in the Figures, but can be readilydeveloped from the above description in accordance with well knowntechniques by those of ordinary skill in the art.

Boat 22 and Cassette 24 Detailed Description

In the preferred embodiment of the invention, the boat 22 is not rotatedduring its cycle of movement around the machine, and hence the generalorientation of the boat 22 is unchanged. Since the boat is moved alongfour sides of a rectangle in the preferred embodiment, the boat 22 ispreferably given a rectangular shape with four sides, each side having acomplimentary surface for engaging one of the four paddles 38A-D in thedrive system.

The boat 22 and cassette 26 are shown in several views in FIGS. 5-8. Theboat and cassette are separate units in the preferred embodiment, withthe boat 22 typically remaining in the machine 20 after processing ofthe cards in the cassette 26 (except for removal for cleaning). Thecassette 26 is loaded with cards 28 and test tubes 30 off line, andplaced in the machine 20 by a technician at the start of the entireprocessing procedure. In an alternative embodiment, the boat andcassette could be an integral unitary sample holder that is removed fromthe machine 20 for loading with cards and test tubes, and placed in tothe loading station of the machine for subsequent processing.

Referring to FIGS. 5-8, the boat 22 has a paddle engaging surface 60Awhich is slanted at an angle relative to the side wall 81 of the boat22. This planar surface 60A is engaged with a complimentary planarangled surface of the head of the paddle 38A as the paddle slides theboat over the front side of the base pan 24. A second angled engagingsurface 60AA is provided, which allows the paddle 38A (FIG. 3) to engagea second surface on the boat 22. This second engaging surface 60AAreduces the collar travel on the shaft 42A needed to move the boat 22 tothe extreme front left corner of the base pan 24. The paddle 38A may berotated out of engagement with surface 60A, moved down the shaft to aposition adjacent to surface 60AA, and rotated back into engagement withsurface 60AA.

The right hand side of the boat 22 has a surface 60D that is engaged bythe paddle 38D when the paddle 38D moves the boat along the right handside of the base pan 24. Similar engaging surfaces are provided on therear and left hand sides of the boat 22.

The cassette 26 has a plurality of slots 61 for sample cards 28 (FIG.1), each of which is adjacent to a test tube holding slot 62 securelyretaining a test tube. A tang 64 or other suitable resilient memberextends inwardly in the test tube holding slot 62 and prevents the testtubes from moving in the slot 62. The card slots 61 are separated fromeach other by a wall 70. The slots 61 are given a dimension so as topermit a slight amount of fore and aft movement of the sample cardsbetween adjacent walls 70. The walls 70 extend only about a third of theway up the cards 28, permitting fore and aft rocking motion of the cardsby the separation device 94 (see FIG. 1), allowing a bar code reader toread bar codes placed at the top of the cards.

The cards 28 rest on the floor 66 of the slots 61. An open side 68 inthe slots permits the cards to be slid out of the cassette 26 into theincubation station 600 (FIG. 1).

Referring to FIG. 5, the boat 22 has a fiat floor 74 which contains anyspills from the test tubes. The floor 74 is given a shape so as tosnugly receive the cassette 26 when the cassette 26 is loaded into theboat 22.

The boat 22 has a substantially flat top planar surface 23 that issupported by a plurality of reinforcing ribs 76 along the sides andbottom of the boat 22. The surface 23 sealingly engages the bottomsurface of the vacuum chamber 402 (FIG. 2). The ribs 76 help the boat 22bear the compressive forces placed on the peripheral sealing surface 23of the boat 22 by the chamber 402.

Referring to FIG. 9, a pair of apertures 78 are provided in the rear ofthe cassette 26 which receive touch buttons (not shown). The touchbuttons identify the contents of the cards 28 loaded into the cassette26. This information is then read by touch button readers 84 mounted tothe center mount 34 of the machine 20 (see FIG. 1). Preferably, astand-alone cassette identification station is provided for the machine20. The station has a computer terminal and touch button contacts thatload information as to the cards for the cassette onto the touch buttonsplaced in the touch button apertures 78.

From the foregoing, persons of ordinary skill in the art will appreciatethat many variations may be made to the mechanical details of thepreferred embodiments without departure from the true spirit and scopeof the invention. For example, the details of the drive system maychange depending on the shape and size of the boat, the geometry of thetesting machine, and the type of stations that are employed in themachine. The configuration of the boat and base pan may also dictate theshape of the paddles and the guide rails in the base pan. The truespirit and scope of the invention is defined by the appended claims, tobe interpreted in light of the foregoing.

We claim:
 1. A system for moving a sample holder within a machine,comprising:a base pan having first, second, third and fourth sideregions, said sample holder placed on and moved about said machine onsaid base pan; a first drive means engageable with said sample holderfor moving said sample holder along a first direction along said basepan aligned with said first side region; a second drive means engageablewith said sample holder for moving said sample holder along a seconddirection along said base pan aligned with said second side region; athird drive means engageable with said sample holder for moving saidsample holder along a third direction along said base pan aligned withsaid third side region; a fourth drive means engageable with said sampleholder for moving said sample holder along a fourth direction along saidbase pan aligned with said fourth side region; said first, second, thirdand fourth drive means independently operable so as to move said sampleholder about said base pan sequentially along said first, second, thirdand fourth side regions and without requiring the presence of othersample holders on said base pan to effectuate the movement of saidsample holder about said first, second, third and fourth side regions ofsaid base pan.
 2. The system of claim 1, wherein said first, second,third and fourth side regions of said base pan each comprise an edgeportion, said edge portions forming four sides of a rectangle, whereinsaid second direction and fourth directions are perpendicular to saidfirst and third directions.
 3. The system of claim 2, wherein saidsample holder comprises a rectangular base and first, second, third andfourth side panels, and wherein said first, second, third and fourthdrive means engage said first, second, third and fourth side panels,respectively, to thereby move said sample holder about said base pan. 4.The system of claim 3, wherein said first and third side panels of saidsample holder comprise an engaging surface for engaging a complementaryengaging surface of said first and third drive means, respectively,enabling said first and third drive means to slide said sample holderover said base pan.
 5. The system of claim 1, wherein said base panfurther comprises at lease one track section comprising at least oneraised ridge for guiding the movement of said sample holder along saidbase pan.
 6. The system of claim 1, wherein said base pan comprisesfirst, second, third and fourth track sections along said first, second,third and fourth side regions, each of said track sections comprising atleast one raised ridge for guiding the movement of said sample holderalong said base pan.
 7. The system of claim 1, wherein said base pancomprises a substantially flat planar surface made from alow-coefficient of sliding friction and low wear material.
 8. The systemof claim 1, wherein said base pan further comprises at least one drainhole.
 9. The system of claim 1, wherein at least one of said first,second, third and fourth drive means is operable in both forward andreverse directions so as to enable movement of said sample holder inforward and reverse direction relative to a respective first, second,third or fourth side region of said base pan.
 10. The system of claim 1,wherein each of said first, second, third and fourth drive meanscomprise (a) a paddle engaging said sample holder moveably mounted to ashaft, the shaft axially fixed with respect to said base ban andextending parallel to said respective first, second third and fourthside regions of said base pan, and (b) means for sliding said paddlerelative to said shaft.
 11. The system of claim 10, wherein said shaftand paddle are rotatable about an axis relative to said base pan as aunit to allow said paddle to be rotated into and out of engagement withsaid first, second, third and fourth side panels of said sample holder,respectively.